Both Hi–maize and chitosan provided better protection of probiotics after exposure of the moist microparticles to simulated gastric and intestinal juice, with counts of 6.35 log CFU g −1, while lower counts were observed for the freeze-dried microcapsules. In contrast, the freeze-dried microparticles of alginate and alginate + Hi–Maize + chitosan had diameters of 114.51 μm and 112.50 μm, respectively. The addition of prebiotics and chitosan increased the size of the moist particles, whose diameter was 70.37 μm, while the diameter of the microparticles containing alginate alone was 55.13 μm. Moist and freeze-dried microparticles were analyzed. Resistant starch (Hi maize) and chitosan at concentrations of 1% and 0.4% were added to the microencapsulation of Lactobacillus acidophilus in alginate beads by extrusion technique.
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